《植物生理学报》 2017, 53(7): 1251-1258

桃PpSnRK1βγ1在拟南芥中超表达提高植株氧化胁迫耐性

赵永飞, 陈晓璐, 彭福田^*, 罗静静, 肖元松

山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018

通信作者：彭福田；E-mail: pft@sdau.edu.cn

摘　要：

以超表达桃(Prunus persica) SnRK1调节亚基βγ编码基因PpSnRK1βγ1的拟南芥(Arabidopsis thaliana)为试材, 使用甲基紫精(MV)模拟氧化胁迫条件, 探讨超表达PpSnRK1βγ1对拟南芥植株抗氧化能力的影响及响应机制。结果表明, 在0.1 μmol·L^-1 MV氧化胁迫条件下, 超表达植株与野生型种子的萌发和根系生长均受到抑制, 但前者具有较高的萌发率和较长的主根; 2~8 μmol·L^-1 MV喷施拟南芥植株叶片, 超表达植株保留更多的绿色叶片。在2 μmol·L^-1 MV喷施3 h后, 超表达植株丙二醛(MDA)含量显著低于野生型, 四种抗氧化酶过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽S-转移酶(GSTs)和过氧化物酶(POD)活性均高于野生型植株, 说明超表达PpSnRK1βγ1基因能提高拟南芥植株的抗氧化能力。在氧化胁迫条件下, 超表达植株的SnRK1酶活性高于野生型植株, 定量PCR结果显示, 在超表达PpSnRK1βγ1拟南芥中, 胁迫响应基因AtHSPRO1和AtHSPRO2的表达量也均较野生型植株明显升高。因此, 超表达PpSnRK1βγ1能够增强拟南芥的抗氧化能力, 可能是通过PpSnRK1βγ1参与HSPRO基因的表达及抗氧化酶活性调控发挥作用的。

关键词：桃; PpSnRK1βγ1; 氧化胁迫; 抗氧化酶; HSPRO

收稿：2016-12-14   修定：2017-04-05

资助：国家自然科学基金(31672099)、山东省“双一流”建设奖补资金(SYL2017YSTD10)和国家现代农业产业技术体系建设专项资金(CARS-31)。

Over expression of a peach PpSnRK1βγ1 gene improving oxidative stress tolerance in Arabidopsis thaliana

ZHAO Yong-Fei, CHEN Xiao-Lu, PENG Fu-Tian^*, LUO Jing-Jing, XIAO Yuan-Song

College of Horticulture Science and Engineering, Shandong Agricultural University / State Key Laboratory of Crop Biology, Taian, Shandong 271018, China

Corresponding author: PENG Fu-Tian; E-mail: pft@sdau.edu.cn

Abstract:

PpSnRK1βγ1, one of the regulatory subunits of SnRK1 (sucrose non-fermenting 1-related kinase 1) in peach, was overexpressed in Arabidopsis thaliana and was used to investigate the function of PpSnRK1βγ1 on oxidative stress tolerance of A. thaliana. Compared to wide type (WT) plants, PpSnRK1βγ1-overexpressing plants showed better seed germination rate and relatively longer root length under oxidative stress. After treated with 2 μmol·L^-1 methyl viologen (MV) for 3 h, compared to WT, MDA contents in transgenic plants were significantly lower, and the activities of CAT (catalase), SOD (superoxide dismutase), GSTs (glutathione S-transferases) and POD (peroxidase) were higher, which indicated that overexpressing PpSnRK1βγ1 could improve the oxidative stress tolerance of A. thaliana. Besides, under oxidative stress condition, the activity of SnRK1 in transgenic plants was more than two times higher than WT. The relative expressions of AtHSPRO1 and AtHSPRO2, the stress-responsive genes, were 2.56 and 3.12 times higher than WT. Therefore, PpSnRK1βγ1 could improve the oxidative stress tolerance of A. thaliana via participating in regulating the expression of HSPRO.

Key words: peach; PpSnRK1βγ1; oxidative stress; antioxidant enzymes; HSPRO